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rabbit anti asc al177 polyclonal antibody  (Adipogen)

 
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    Adipogen rabbit anti asc al177 polyclonal antibody
    Rabbit Anti Asc Al177 Polyclonal Antibody, supplied by Adipogen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+asc+al177+polyclonal+antibody/antibody+asc+polyclonal+rabbit+to/pmc12484792-377-34-39
    Average 86 stars, based on 1 article reviews
    rabbit anti asc al177 polyclonal antibody - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Macrophage WDFY3 mitigates autoimmunity by enhancing efferocytosis and suppressing T cell activation in mice
    Article Snippet: .. Permeabilization was performed using 0.5% Triton X-100 in PBS, followed by blocking with 5% BSA in PBST for 30 min. For ASC staining , the cells were incubated overnight at 4 °C with a rabbit anti-ASC (AL177) polyclonal antibody (AdipoGen, AG-25B-0006-C100, 1:50), followed by 1 h incubation at room temperature with an AF488 goat anti-rabbit IgG (H + L) secondary antibody (Invitrogen, A11008, 1:200). .. Nuclei were counterstained with DAPI (Invitrogen, 62248, 1:10,000).

    Staining:

    Article Title: Macrophage WDFY3 mitigates autoimmunity by enhancing efferocytosis and suppressing T cell activation in mice
    Article Snippet: .. Permeabilization was performed using 0.5% Triton X-100 in PBS, followed by blocking with 5% BSA in PBST for 30 min. For ASC staining , the cells were incubated overnight at 4 °C with a rabbit anti-ASC (AL177) polyclonal antibody (AdipoGen, AG-25B-0006-C100, 1:50), followed by 1 h incubation at room temperature with an AF488 goat anti-rabbit IgG (H + L) secondary antibody (Invitrogen, A11008, 1:200). .. Nuclei were counterstained with DAPI (Invitrogen, 62248, 1:10,000).

    Incubation:

    Article Title: Macrophage WDFY3 mitigates autoimmunity by enhancing efferocytosis and suppressing T cell activation in mice
    Article Snippet: .. Permeabilization was performed using 0.5% Triton X-100 in PBS, followed by blocking with 5% BSA in PBST for 30 min. For ASC staining , the cells were incubated overnight at 4 °C with a rabbit anti-ASC (AL177) polyclonal antibody (AdipoGen, AG-25B-0006-C100, 1:50), followed by 1 h incubation at room temperature with an AF488 goat anti-rabbit IgG (H + L) secondary antibody (Invitrogen, A11008, 1:200). .. Nuclei were counterstained with DAPI (Invitrogen, 62248, 1:10,000).

    Article Title: Macrophage WDFY3 mitigates autoimmunity by enhancing efferocytosis and suppressing T cell activation in mice.
    Article Snippet: .. For ASC staining70, the cells were incubated overnight at 4 °C with a rabbit anti-ASC (AL177) polyclonal antibody (AdipoGen, AG-25B0006-C100, 1:50), followed by 1 h incubation at room temperature with an AF488 goat anti-rabbit IgG (H + L) secondary antibody (Invitrogen, A11008, 1:200). .. Nuclei were counterstained with DAPI (Invitrogen, 62248, 1:10,000).



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    rabbit anti asc al177 polyclonal antibody  (Adipogen)
    86
    Adipogen rabbit anti asc al177 polyclonal antibody
    Rabbit Anti Asc Al177 Polyclonal Antibody, supplied by Adipogen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+asc+al177+polyclonal+antibody/antibody+asc+polyclonal+rabbit+to/pmc12484792-377-34-39
    Average 86 stars, based on 1 article reviews
    rabbit anti asc al177 polyclonal antibody - by Bioz Stars, 2026-10
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    rabbit polyclonal anti-asc (al177)  (Adipogen)
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    Ethanol activates NLRP3 inflammasome in the hippocampus of APP/PS1 mice. Representative image and quantification of NLRP3 ( A ), ASC ( B ), and caspase-1 ( C ) protein levels in the hippocampal tissue lysates from WT and APP/PS1 mice analyzed by Western blot. Mean ± SEM. N = 8–12. D Caspase-1 activity measured in the hippocampal tissue lysates using a fluorescent assay, displayed as a fold change in the fluorescence compared to WT-water group. E IL-1β protein levels in the hippocampus of WT and APP/PS1 mice evaluated by ELISA. Mean ± SEM. N = 8–14. F Quantification of ASC fluorescence staining in the hippocampus. Mean ± SEM. N = 8–10. Two-way ANOVA with Tukey’s post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. G Representative pictures of ASC staining (red) localized around and inside Aβ plaques (green, 6E10 staining) in the hippocampus of water- and ethanol-treated APP/PS1 mice. Scale bar – 50 µm. H Representative pictures of ASC (red, <t>AL177</t> staining) co-localization with microglia (IBA1, green). DAPI-blue. Scale bar – 20 µm. I Representative pictures of ASC (red, AL177 staining) co-localization with astrocytes (GFAP, light blue). DAPI-blue. Scale bar – 20 µm. ● denotes females, ○ denotes males
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    Ethanol activates NLRP3 inflammasome in the hippocampus of APP/PS1 mice. Representative image and quantification of NLRP3 ( A ), ASC ( B ), and caspase-1 ( C ) protein levels in the hippocampal tissue lysates from WT and APP/PS1 mice analyzed by Western blot. Mean ± SEM. N = 8–12. D Caspase-1 activity measured in the hippocampal tissue lysates using a fluorescent assay, displayed as a fold change in the fluorescence compared to WT-water group. E IL-1β protein levels in the hippocampus of WT and APP/PS1 mice evaluated by ELISA. Mean ± SEM. N = 8–14. F Quantification of ASC fluorescence staining in the hippocampus. Mean ± SEM. N = 8–10. Two-way ANOVA with Tukey’s post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. G Representative pictures of ASC staining (red) localized around and inside Aβ plaques (green, 6E10 staining) in the hippocampus of water- and ethanol-treated APP/PS1 mice. Scale bar – 50 µm. H Representative pictures of ASC (red, <t>AL177</t> staining) co-localization with microglia (IBA1, green). DAPI-blue. Scale bar – 20 µm. I Representative pictures of ASC (red, AL177 staining) co-localization with astrocytes (GFAP, light blue). DAPI-blue. Scale bar – 20 µm. ● denotes females, ○ denotes males
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    Ethanol activates NLRP3 inflammasome in the hippocampus of APP/PS1 mice. Representative image and quantification of NLRP3 ( A ), ASC ( B ), and caspase-1 ( C ) protein levels in the hippocampal tissue lysates from WT and APP/PS1 mice analyzed by Western blot. Mean ± SEM. N = 8–12. D Caspase-1 activity measured in the hippocampal tissue lysates using a fluorescent assay, displayed as a fold change in the fluorescence compared to WT-water group. E IL-1β protein levels in the hippocampus of WT and APP/PS1 mice evaluated by ELISA. Mean ± SEM. N = 8–14. F Quantification of ASC fluorescence staining in the hippocampus. Mean ± SEM. N = 8–10. Two-way ANOVA with Tukey’s post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. G Representative pictures of ASC staining (red) localized around and inside Aβ plaques (green, 6E10 staining) in the hippocampus of water- and ethanol-treated APP/PS1 mice. Scale bar – 50 µm. H Representative pictures of ASC (red, <t>AL177</t> staining) co-localization with microglia (IBA1, green). DAPI-blue. Scale bar – 20 µm. I Representative pictures of ASC (red, AL177 staining) co-localization with astrocytes (GFAP, light blue). DAPI-blue. Scale bar – 20 µm. ● denotes females, ○ denotes males
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    Ethanol activates NLRP3 inflammasome in the hippocampus of APP/PS1 mice. Representative image and quantification of NLRP3 ( A ), ASC ( B ), and caspase-1 ( C ) protein levels in the hippocampal tissue lysates from WT and APP/PS1 mice analyzed by Western blot. Mean ± SEM. N = 8–12. D Caspase-1 activity measured in the hippocampal tissue lysates using a fluorescent assay, displayed as a fold change in the fluorescence compared to WT-water group. E IL-1β protein levels in the hippocampus of WT and APP/PS1 mice evaluated by ELISA. Mean ± SEM. N = 8–14. F Quantification of ASC fluorescence staining in the hippocampus. Mean ± SEM. N = 8–10. Two-way ANOVA with Tukey’s post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. G Representative pictures of ASC staining (red) localized around and inside Aβ plaques (green, 6E10 staining) in the hippocampus of water- and ethanol-treated APP/PS1 mice. Scale bar – 50 µm. H Representative pictures of ASC (red, <t>AL177</t> staining) co-localization with microglia (IBA1, green). DAPI-blue. Scale bar – 20 µm. I Representative pictures of ASC (red, AL177 staining) co-localization with astrocytes (GFAP, light blue). DAPI-blue. Scale bar – 20 µm. ● denotes females, ○ denotes males
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    Average 90 stars, based on 1 article reviews
    rabbit anti-human asc polyclonal antibody al177 - by Bioz Stars, 2026-10
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    		  Buy from Supplier
    rabbit polyclonal anti-asc al177  (Adipogen)
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    Adipogen rabbit polyclonal anti-asc al177
    Ethanol activates NLRP3 inflammasome in the hippocampus of APP/PS1 mice. Representative image and quantification of NLRP3 ( A ), ASC ( B ), and caspase-1 ( C ) protein levels in the hippocampal tissue lysates from WT and APP/PS1 mice analyzed by Western blot. Mean ± SEM. N = 8–12. D Caspase-1 activity measured in the hippocampal tissue lysates using a fluorescent assay, displayed as a fold change in the fluorescence compared to WT-water group. E IL-1β protein levels in the hippocampus of WT and APP/PS1 mice evaluated by ELISA. Mean ± SEM. N = 8–14. F Quantification of ASC fluorescence staining in the hippocampus. Mean ± SEM. N = 8–10. Two-way ANOVA with Tukey’s post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. G Representative pictures of ASC staining (red) localized around and inside Aβ plaques (green, 6E10 staining) in the hippocampus of water- and ethanol-treated APP/PS1 mice. Scale bar – 50 µm. H Representative pictures of ASC (red, <t>AL177</t> staining) co-localization with microglia (IBA1, green). DAPI-blue. Scale bar – 20 µm. I Representative pictures of ASC (red, AL177 staining) co-localization with astrocytes (GFAP, light blue). DAPI-blue. Scale bar – 20 µm. ● denotes females, ○ denotes males
    Rabbit Polyclonal Anti Asc Al177, supplied by Adipogen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
    rabbit polyclonal anti-asc al177 - by Bioz Stars, 2026-10
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    rabbit anti-asc rabbit polyclonal pab al177  (Adipogen)
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    Ethanol activates NLRP3 inflammasome in the hippocampus of APP/PS1 mice. Representative image and quantification of NLRP3 ( A ), ASC ( B ), and caspase-1 ( C ) protein levels in the hippocampal tissue lysates from WT and APP/PS1 mice analyzed by Western blot. Mean ± SEM. N = 8–12. D Caspase-1 activity measured in the hippocampal tissue lysates using a fluorescent assay, displayed as a fold change in the fluorescence compared to WT-water group. E IL-1β protein levels in the hippocampus of WT and APP/PS1 mice evaluated by ELISA. Mean ± SEM. N = 8–14. F Quantification of ASC fluorescence staining in the hippocampus. Mean ± SEM. N = 8–10. Two-way ANOVA with Tukey’s post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. G Representative pictures of ASC staining (red) localized around and inside Aβ plaques (green, 6E10 staining) in the hippocampus of water- and ethanol-treated APP/PS1 mice. Scale bar – 50 µm. H Representative pictures of ASC (red, <t>AL177</t> staining) co-localization with microglia (IBA1, green). DAPI-blue. Scale bar – 20 µm. I Representative pictures of ASC (red, AL177 staining) co-localization with astrocytes (GFAP, light blue). DAPI-blue. Scale bar – 20 µm. ● denotes females, ○ denotes males
    Rabbit Anti Asc Rabbit Polyclonal Pab Al177, supplied by Adipogen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+asc+al177+polyclonal+antibody/asc+antibody/pm37945612-272-6-14
    Average 90 stars, based on 1 article reviews
    rabbit anti-asc rabbit polyclonal pab al177 - by Bioz Stars, 2026-10
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    rabbit anti-asc rabbit polyclonal pab al177, 1:200 dilution  (Adipogen)
    90
    Adipogen rabbit anti-asc rabbit polyclonal pab al177, 1:200 dilution
    Ethanol activates NLRP3 inflammasome in the hippocampus of APP/PS1 mice. Representative image and quantification of NLRP3 ( A ), ASC ( B ), and caspase-1 ( C ) protein levels in the hippocampal tissue lysates from WT and APP/PS1 mice analyzed by Western blot. Mean ± SEM. N = 8–12. D Caspase-1 activity measured in the hippocampal tissue lysates using a fluorescent assay, displayed as a fold change in the fluorescence compared to WT-water group. E IL-1β protein levels in the hippocampus of WT and APP/PS1 mice evaluated by ELISA. Mean ± SEM. N = 8–14. F Quantification of ASC fluorescence staining in the hippocampus. Mean ± SEM. N = 8–10. Two-way ANOVA with Tukey’s post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. G Representative pictures of ASC staining (red) localized around and inside Aβ plaques (green, 6E10 staining) in the hippocampus of water- and ethanol-treated APP/PS1 mice. Scale bar – 50 µm. H Representative pictures of ASC (red, <t>AL177</t> staining) co-localization with microglia (IBA1, green). DAPI-blue. Scale bar – 20 µm. I Representative pictures of ASC (red, AL177 staining) co-localization with astrocytes (GFAP, light blue). DAPI-blue. Scale bar – 20 µm. ● denotes females, ○ denotes males
    Rabbit Anti Asc Rabbit Polyclonal Pab Al177, 1:200 Dilution, supplied by Adipogen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+asc+al177+polyclonal+antibody/asc+antibody/pmc10636019-255-6-14
    Average 90 stars, based on 1 article reviews
    rabbit anti-asc rabbit polyclonal pab al177, 1:200 dilution - by Bioz Stars, 2026-10
    90/100 stars
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    anti-asc (al177), rabbit polyclonal ab  (Adipogen)
    90
    Adipogen anti-asc (al177), rabbit polyclonal ab
    Ethanol activates NLRP3 inflammasome in the hippocampus of APP/PS1 mice. Representative image and quantification of NLRP3 ( A ), ASC ( B ), and caspase-1 ( C ) protein levels in the hippocampal tissue lysates from WT and APP/PS1 mice analyzed by Western blot. Mean ± SEM. N = 8–12. D Caspase-1 activity measured in the hippocampal tissue lysates using a fluorescent assay, displayed as a fold change in the fluorescence compared to WT-water group. E IL-1β protein levels in the hippocampus of WT and APP/PS1 mice evaluated by ELISA. Mean ± SEM. N = 8–14. F Quantification of ASC fluorescence staining in the hippocampus. Mean ± SEM. N = 8–10. Two-way ANOVA with Tukey’s post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. G Representative pictures of ASC staining (red) localized around and inside Aβ plaques (green, 6E10 staining) in the hippocampus of water- and ethanol-treated APP/PS1 mice. Scale bar – 50 µm. H Representative pictures of ASC (red, <t>AL177</t> staining) co-localization with microglia (IBA1, green). DAPI-blue. Scale bar – 20 µm. I Representative pictures of ASC (red, AL177 staining) co-localization with astrocytes (GFAP, light blue). DAPI-blue. Scale bar – 20 µm. ● denotes females, ○ denotes males
    Anti Asc (Al177), Rabbit Polyclonal Ab, supplied by Adipogen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+asc+al177+polyclonal+antibody/asc+antibody/pm37172565-638-2-11
    Average 90 stars, based on 1 article reviews
    anti-asc (al177), rabbit polyclonal ab - by Bioz Stars, 2026-10
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    Image Search Results


    Ethanol activates NLRP3 inflammasome in the hippocampus of APP/PS1 mice. Representative image and quantification of NLRP3 ( A ), ASC ( B ), and caspase-1 ( C ) protein levels in the hippocampal tissue lysates from WT and APP/PS1 mice analyzed by Western blot. Mean ± SEM. N = 8–12. D Caspase-1 activity measured in the hippocampal tissue lysates using a fluorescent assay, displayed as a fold change in the fluorescence compared to WT-water group. E IL-1β protein levels in the hippocampus of WT and APP/PS1 mice evaluated by ELISA. Mean ± SEM. N = 8–14. F Quantification of ASC fluorescence staining in the hippocampus. Mean ± SEM. N = 8–10. Two-way ANOVA with Tukey’s post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. G Representative pictures of ASC staining (red) localized around and inside Aβ plaques (green, 6E10 staining) in the hippocampus of water- and ethanol-treated APP/PS1 mice. Scale bar – 50 µm. H Representative pictures of ASC (red, AL177 staining) co-localization with microglia (IBA1, green). DAPI-blue. Scale bar – 20 µm. I Representative pictures of ASC (red, AL177 staining) co-localization with astrocytes (GFAP, light blue). DAPI-blue. Scale bar – 20 µm. ● denotes females, ○ denotes males

    Journal: Journal of Neuroinflammation

    Article Title: Ethanol consumption aggravates amyloid pathology and neuroinflammation in Alzheimer’s disease associated with inflammasome activation and ASC speck propagation

    doi: 10.1186/s12974-025-03501-8

    Figure Lengend Snippet: Ethanol activates NLRP3 inflammasome in the hippocampus of APP/PS1 mice. Representative image and quantification of NLRP3 ( A ), ASC ( B ), and caspase-1 ( C ) protein levels in the hippocampal tissue lysates from WT and APP/PS1 mice analyzed by Western blot. Mean ± SEM. N = 8–12. D Caspase-1 activity measured in the hippocampal tissue lysates using a fluorescent assay, displayed as a fold change in the fluorescence compared to WT-water group. E IL-1β protein levels in the hippocampus of WT and APP/PS1 mice evaluated by ELISA. Mean ± SEM. N = 8–14. F Quantification of ASC fluorescence staining in the hippocampus. Mean ± SEM. N = 8–10. Two-way ANOVA with Tukey’s post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. G Representative pictures of ASC staining (red) localized around and inside Aβ plaques (green, 6E10 staining) in the hippocampus of water- and ethanol-treated APP/PS1 mice. Scale bar – 50 µm. H Representative pictures of ASC (red, AL177 staining) co-localization with microglia (IBA1, green). DAPI-blue. Scale bar – 20 µm. I Representative pictures of ASC (red, AL177 staining) co-localization with astrocytes (GFAP, light blue). DAPI-blue. Scale bar – 20 µm. ● denotes females, ○ denotes males

    Article Snippet: Rabbit polyclonal anti-ASC (AL177) , Adipogen , AG-25B-0006-C100.

    Techniques: Western Blot, Activity Assay, Fluorescence, Enzyme-linked Immunosorbent Assay, Staining